Three-dimensional view of a selectin cell adhesion molecule.

glycans in the L2/HNK-1 carbohydrate epitope expressing neural adhesion molecule PO. Demonstration that a lectin-like receptor (Gp90Mel) directly mediates adhesion of lymphocytes to high endothelial veaules of lymph nodes. The neural cell adhesion molecule N-CAM enhances LI-dependent cell cell interactions. (1989) Specificity of binding of three soluble rat lung lectins to substituted and unsubstituted mammalian 0-galactosides. Malignant Cells have increased levels of common glycoprotein ligands of the endogenous cerebellar soluble lectin. Eur. Evidence for heterophilic adhesion of embryonic retinal cells and neurOblastoma cells to substratum-adsorbed NCAM. Equilibrium binding analysis of neural cell adhesion molecule binding to heparin. (1992) Identification Of a peptide sequence involved in homophilic binding in the neural cell adhesion molecule NCAM. (1990) A lymphocyte homing receptor is a lectin a member of the emerging LEC-CAM family. Gtytobiobgy, 1, 69-90. The neural cell adhesion molecule (N-CAM) as a regulator of cell cell interactions. peripheral myelin protein P0 confers both adhesion and fteurlte outgrowth' promoting properties. The sensation and regulation of interactions With the extracellular environment. The cell biology of lymphocyte adhesion receptors. immunochemical study of A soluble cerebellar lectin delineating its structure and function. (1991) Malignant transformation in hepatocytes is associated with the general increase Of glycoprotein ligands Specifically binding to the endo-genous lectin CSL. Introduction The Selectin cell adhesion molecules provide our best current paradigm for the role of sugar-lectin interactions in biological recognition. The three knowh members of this group mediate initial, weak interaction between leukocytes and vascular endothelial cells, As a result of this first recognition event, blood cells roll along the surface of the epithelium, where subsequent protein-protein interactions lead to extravasation (Springer, 1994). E-and P-selectin on endothelial cells, particularly at sites of inflammation, bind carbohydrate determinants on monocytes and neutrophils, while L-selectin on Circulating T lymphocytes binds to high endothelial venules in the peripheral lymph nodes to which they home. Determination of the three-dimensional structure of a portion of the E-selectin molecule by Graves et al. (1994) represents an important step forward in our understanding of how these molecules function at the atomic level. The selectins contain three types of extracellular protein modules (Lasky, 1992). In each protein, an N-terminal carbohydrate recognition domain (CRD) is adjacent to an epidermal growth factor (EGPVlike repeat. These domains are connected to the membrane through a string of 2 "-9 repeats of a consensus repeat module Of the type found initially in complement-binding proteins. Graves etal. undertook …